- Iolaus and Hercules have known each other their whole lives, but were not always close friends (Young Hercules (Movie Pilot))
- Was promised a pet dog by his father, but never got one. (1.18 - A Serpent's Tooth)
- He did, however, have a pet goat. (1.07 - Inn Trouble)
- Iolaus grew up as a thief, and ran with the Lowacks - one of the most dangerous gangs around. (1.04 - Herc and Seek)
- When Iolaus was caught, he was given the option to go to jail or go to Cheiron's Academy. Although he chose the Academy, Iolaus continues to struggle to pass the exams and earn enough money to pay tuition. (See: Jail or Cheiron's Academy)
Iolaus typically wears a patchwork vest and brown leather pants, although he appears shirtless in several episodes.
- Has excellent aim - 1.01 - Treasure of Zeus, 1.05 - Girl Trouble.
- Can climb rope extremely fast (at the speed of gravity vs his own body weight) - 1.02 - Between Friends.
- Escape Artist - Starts to chew his way through the ropes around his wrists - 1.04 - Herc and Seek, somehow frees himself after hanging upside-down from a chain in the stables - 1.13 - Forgery.
- The Ol'Iolaus Charm (Not quite as powerful as Iolaus likes to think it is) - 1.05 - Girl Trouble.
- Pickpocket - 1.05 - Girl Trouble.
- Food Service Experience - 1.06 - Teacher's Pests, 1.07 - Inn Trouble.
- Artistic - Created the Pink Cart of Femininity presumably all by himself - 1.14 - No Way Out.
- Fishing - 1.15 - Ares on Trial, 1.16 - Down and Out in Academy Hills.
- Can sing - 1.42 - The Prize.
- Lilith - (see Iolaus and Lilith).
- Kora - (see Iolaus and Kora).
- Sabrina - (1.10 - Cyrano de Hercules).
- Corinth Girl Rachel - (1.13 - Forgery).
- Bacchae Katherine - (1.19 - The Lure of the Lyre).
- Teenage Fan Victoria, Bar Girl Serena - (1.20 - Fame).
- Princess Dido - (1.35 - My Fair Lilith).
- In 1.42 - The Prize, a random girl shouts out "I love you, Iolaus!"
- Mila - (1.47 - Mila).
- After learning that Mila is headed to Cheiron's Academy, Iolaus introduces himself and Hercules as "the star cadets". He offers his hand to Mila to shake, but she rejects him with a repulsed look. He recognizes her name and mentions that he knows about her winning the Young Amazon Competitions. When she asks how he knows about that, he explains that he was an honor guest of Cyane's tribe, and it practically an amazon himself. But Mila is much more interested in Hercules, and asks him to show her the way to the Academy. As they walk away chatting, Iolaus sighs, "That is so typical."
- After Mila mentions the weakness in Panthar's fighting style that allows Iolaus to defeat him, Iolaus wags his finger at her and says, "Looking out for me already, huh? And we haven't even gone out yet." She responds, "No offense, but I'm not here to have fun. I'm here to fight." Iolaus growls in disappointment.
- As Mila is leaving, Iolaus tells her, "If you ever feel any need for Amazon bonding, you know where to find me." Mila grins, "He just never gives up, does he?" "Nope," Hercules admits. "Now that's my kind of guy," she says, slapping Iolaus's face lightly. As she walks away, Hercules and Iolaus both sigh, "Amazons."
- Really hates those slimy things that live in caves - 1.01 - Treasure of Zeus
- Cannot swim - 1.13 - Forgery, 1.15 - Ares on Trial, 1.18 - A Serpent's Tooth
- Cannot cook - 1.05 - Girl Trouble
- Severely hydrophobic - 1.28 - In Your Dreams
- Hates asparagus - 1.31 - Home for the Holidays
- See Iolaus Is Always Eating page
- See Jason and Iolaus for a breakdown of their friendship
- See Hercules and Iolaus for a breakdown of their friendship